sima cell line
Split confluent culture 13 to 15 every 3-6 days using trypsinEDTA or by tapping the flask. To give you confidence in the health of your cells every step of the way weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. Sima Lev Metastasis Drug Resistance Cell Culture Differentiation and Generation of VAMP2 SiMa Cell Lines. . Established from the adrenal tumor tissue resected after treatment from a 20-month-old boy of origin with neuroblastoma stage III in 1991. To be able to keep the cells healthy for seven days without sub-culturing them all three differentiation protocols were performed in combination with serum deprivation. No matter how you are using your. The plates were maintained for 2 weeks at which time an unexpected. Human neuroblastoma cell lines SiMa and IMR-32 were treated for seven days with growth supplements B-27 and N-2 all-trans-retinoic ac...